Article
RNase 7 – part of the innate immune system of the nasal mucosa
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Published: | July 6, 2010 |
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Introduction: Ribonuclease (RNase) 7 is a cationic peptide with broad antimicrobial activity including Staphylococcus (S.) aureus and is detectable e.g. in epithelia of the skin and intestine. Hitherto nasal secretion has not been investigated in respect to RNase 7 concentration although inhaled microorganisms have first contact with the immune system in the nasal cavity.
Patients and methods: The RNase 7 concentration in nasal secretions of patients with Wegeners Granulomatosis (WG, n=12), Polyposis nasi (PP, n=11) and healthy controls (n=12) as well as RNase 7 concentration in the serum of 14 WG, 12 PP and 13 healthy controls was analyzed by ELISA. Primary nasal epithelial cell cultures (NEC) of healthy volunteers were stimulated with S. aureus and the concentration of RNase 7 in the supernatants have been determined.
Results: RNase 7 was detectable in nasal secretions of all three groups. The differences were not significant. In serum RNase 7 was verified in 14 of 35 probes and uniformly distributed among the groups. After stimulation of NEC with increasing concentrations of S. aureus the RNase 7 concentration in the supernatant decreased.
Discussion: RNase 7 is part of the innate immune system of the nasal mucosal barrier and rarely detectable systemically. Referring to our data no defect of the nasal barrier was detectable for WG and PP. An inhibiting effect for increasing concentrations of bacteria seems functionally possible. Further investigation particularly regarding possible interactions between microorganisms and epithelial cells (bacterial peptidases, inhibiting components in nasal secretion, interaction of antimicrobial peptides) will follow.
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