Article
Ultrasound-based growth analysis of cultured chondrocyte aggregates
Search Medline for
Authors
Published: | July 6, 2010 |
---|
Outline
Text
Introduction: The subject of the present study is the in vivo growth behavior of in vitro cultured chondrocyte aggregates. The aim of this study was a repetitive, non-invasive volume determination of engineered cartilage tissue based on human chondrocytes in a mouse model by using 30 MHz ultrasound technology.
Methods: Cartilage cells were isolated from human nasal septum cartilage (n=10; m=6/w=4) and expanded for 3 weeks in a monolayer culture. Then the isolated chondrocytes were cultured for further 3 weeks in an in vitro aggregate culture system before 48 aggregates were implanted subcutaneously in an athymic nude mouse model for 4 and 8 weeks respectively. The aggregate volume was analyzed over time by using the high-resolution, 3-D ultrasound imaging system Vevo770TM (Visual Sonics, Toronto, ON, Canada).
Results: First results of collected data showed both, donor-site specific differences in aggregate volumes during in vivo cultivation as well as volume variations within a collective over the 8-week period of study.
Conclusion: The documented volume variations of specific cartilage specimen in 3D aggregates show that, based on a constant number of cells, there are variations in regard to in vivo behavior of individual transplants of in vitro cultured cartilage.
Supported by: HomFor