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23rd Annual Meeting of the German Retina Society

German Retina Society

24.09. - 25.09.2010, Freiburg

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Retinal microglia in the mouse model of oxygen-induced retinopathy

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  • Franziska Fischer - University Eye Clinic Freiburg i. Br.
  • G. Martin - University Eye Clinic Freiburg i. Br.
  • H. Agostini - University Eye Clinic Freiburg i. Br.

German Retina Society. 23rd Annual Conference of the German Retina Society. Freiburg i. Br., 24.-25.09.2010. Düsseldorf: German Medical Science GMS Publishing House; 2010. Doc10rg44

doi: 10.3205/10rg44, urn:nbn:de:0183-10rg441

This is the English version of the article.
The German version can be found at: http://www.egms.de/de/meetings/rg2010/10rg44.shtml

Published: September 21, 2010

© 2010 Fischer et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

Outline

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Background and Purpose: Retinal neovascularization has been intensively investigated in the mouse model of oxygen-induced retinopathy (OIR). But the role of microglial cells (MC) in oxygen induced retinopathy with cell loss and recative angiogenesis has not yet been clarified.

Methods: Mice with the Cx3cr1 gene replaced by GFP were used to identify MC. They were kept in 75% oxygen from post-natal day 7 (P7) to P12. After return to normal room air, retinal flat-mounts or cryosections were stained with lectin and investigated by fluorescence microscopy.

Results: MC are not activated in the fully developed, healthy retina. In the OIR model, activated microglia were observed twice: during the hyperoxic phase (P10) and during neovasularisation (P17). The density of resting MC in the deep layer decreases significantly after return to normal room air.

Conclusions: Using Cx3cr1-deficient GFP-positive mice, it is possible to study the role of MC in vascular remodeling. Activated MC may be involved in the formation of the central avascular zone as well as in retinal revascularisation.