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62nd Annual Meeting of the German Society of Neurosurgery (DGNC)
Joint Meeting with the Polish Society of Neurosurgeons (PNCH)

German Society of Neurosurgery (DGNC)

7 - 11 May 2011, Hamburg

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Podoplanin contributes to migration and angiogenesis in malignant glioma in vitro

Meeting Abstract

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  • S. Grau - Department of Neurosurgery, Ludwig-Maximilian-University Munich; Department of Neurosurgery, University of Cologne, Germany
  • F. Trillsch - Department of Gynaecology, University Medical Center, Hamburg-Eppendorf
  • J.C. Tonn - Department of Neurosurgery, Ludwig-Maximilian-University Munich
  • R. Goldbrunner - Department of Neurosurgery, University of Cologne, Germany
  • S. Fugger - Department of Neurosurgery, Ludwig-Maximilian-University Munich
  • E. Noessner - Helmholtz Zentrum Munich
  • P. Nelson - Department of internal Medicine, Ludwig-Maximilian-University Munich
  • I. von Luettichau - Department of Pediatrics, Technical University Munich

Deutsche Gesellschaft für Neurochirurgie. Polnische Gesellschaft für Neurochirurgen. 62. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC), Joint Meeting mit der Polnischen Gesellschaft für Neurochirurgen (PNCH). Hamburg, 07.-11.05.2011. Düsseldorf: German Medical Science GMS Publishing House; 2011. DocP 027

doi: 10.3205/11dgnc248, urn:nbn:de:0183-11dgnc2483

Published: April 28, 2011

© 2011 Grau et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

Outline

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Objective: Podoplanin has been shown to be widely expressed in a variety of human tumors. In brain tumors, podoplanin is expressed in a grade-dependent manner. While this protein has been shown to be involved in tumor metastasis, little is known about its biological function in gliomas. Here, we describe our investigation of the biology of podoplanin in human glioma cells.

Methods: Human glioma cell lines (U373MG and U87MG) were stably transfected with expression plasmids encoding podoplanin. Subsequent influence on cell proliferation, migration and invasion were assessed by MTT, collagen sprouting, spheroid confrontation and on tube formation induction in co-incubation studies with endothelial cells using matrigel tube formation assay.

Results: Podoplanin expression was confirmed by FACS analysis, PCR and immunocytochemistry. Cells were sorted for highly podoplanin expressing cells (U373Phigh/U87Phigh). Wild type (wt) and mock transfected U373 were podoplanin negative, while wtU87 showed low basal expression (U373Pmock/U87Pmock). Transfection did not influence the production of pro-angiogenic factors including VEGF, VEGF-C and D. Expression of VEGF receptors (VEGFR) remained unchanged except for U87Phigh, for which VEGFR3 expression was induced. U373Phigh showed significantly reduced proliferation as compared to the mock transfected group. In contrast, podoplanin significantly increased migration in the collagen matrix. Furthermore, human brain microvascular endothelial cells showed stronger tube formation on matrigel when cultured in conditioned media from Phigh glioma cells.

Conclusions: Podoplanin expression leads to the increased migration of tumor cells and enhances tube formation activity in human brain endothelial cells independently of VEGF.