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49. Jahrestagung der Österreichischen Gesellschaft für Plastische, Ästhetische und Rekonstruktive Chirurgie (ÖGPÄRC), 42. Jahrestagung der Deutschen Gesellschaft der Plastischen, Rekonstruktiven und Ästhetischen Chirurgen e. V. (DGPRÄC), 16. Jahrestagung der Vereinigung der Deutschen Ästhetisch-Plastischen Chirurgen e. V. (VDÄPC)

29.09. - 01.10.2011, Innsbruck

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A new design for in-vivo-tissue engineering of musculoskeletal tissue using the inferior epigastric artery central anastomosable vessel of a 3-dimensional construct

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  • author Sebastian Erol Dunda - Klinik für Plastische Chirurgie, Hand- und Verbrennungschirurgie, UK Aachen
  • Teresa Schriever - Klinik für Plastische Chirurgie, Hand- und Verbrennungschirurgie, UK Aachen
  • Christian Rosen - Institut für Anatomie, UK Aachen
  • Stefanos Diamantouros - Helmholtz-Institut, Biomedical Engineering, RWTH Aachen
  • Stefan Jockenhövel - Helmholtz-Institut, Biomedical Engineering, RWTH Aachen
  • Norbert Pallua - Klinik für Plastische Chirurgie, Hand- und Verbrennungschirurgie, UK Aachen

Österreichische Gesellschaft für Plastische, Ästhetische und Rekonstruktive Chirurgie. Deutsche Gesellschaft der Plastischen, Rekonstruktiven und Ästhetischen Chirurgen. Vereinigung der Deutschen Ästhetisch-Plastischen Chirurgen. 49. Jahrestagung der Österreichischen Gesellschaft für Plastische, Ästhetische und Rekonstruktive Chirurgie (ÖGPÄRC), 42. Jahrestagung der Deutschen Gesellschaft der Plastischen, Rekonstruktiven und Ästhetischen Chirurgen (DGPRÄC), 16. Jahrestagung der Vereinigung der Deutschen Ästhetisch-Plastischen Chirurgen (VDÄPC). Innsbruck, 29.09.-01.10.2011. Düsseldorf: German Medical Science GMS Publishing House; 2011. Doc11dgpraecP97

doi: 10.3205/11dgpraec281, urn:nbn:de:0183-11dgpraec2819

Published: September 27, 2011

© 2011 Dunda et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

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Introduction: The creation of musculoskeletal tissue represents an alternative for the replacement of tissue after severe damage. However, most of the approaches of creating musculoskeletal tissue have their limitations in the size as the maximally obtainable dimensions of bioartificial tissue are limited due to lack of supporting vessels within the 3-dimensional-construct. The seeded myoblasts require high amounts of perfusion, oxygen and nutrients to survive. To achieve this, we developed a transplantable 3-dimensional-scaffold which features a macroscopic core vessel.

Materials and methods: In animal studies on Wistar rats the inferior epigastric artery was found to be suitable for implantation of an in-vivo bioreactor chamber. After dissecting the epigastric artery with leaving the small branches open the customized chamber was implanted hosting the inferior epigastric artery as central core vessel. Fibrin glue was administered as matrix surrounding the artery in the bioreactor. Luciferase-transfected myoblasts were implemented and followed up by bioluminescence for a period of 7 days.

Results: The implantation of the in-vivo-bioreactor with the epigastric artery as core vessel was done routinely without any side effects or wound problems during the experimental period. The follow-up of the implanted myoblasts by bioluminescence showed a higher cell survival in areas of the open branches of the inferior epigastric artery.

Conclusions: Again, it was shown that the cell survival in-vivo is highly depending on the blood supply. The inferior epigastric artery is easily accessible and therefore usable as a core vessel for in-vivo tissue-engineering in the rat model offering a wide range of further experimental approaches.