gms | German Medical Science

24th Annual Meeting of the German Retina Society

German Retina Society

17.06. - 18.06.2011, Aachen

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In-vivo and ex-vivo characterization of laser-induced choroidal neovascularization variability in mice

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  • Robert Hörster - Universitäts-Augenklinik Köln
  • P.S. Müther - Universitäts-Augenklinik Köln
  • S. Vierkotten - Universitäts-Augenklinik Köln
  • S. Schröder - Universitäts-Augenklinik Köln
  • B. Kirchhof - Universitäts-Augenklinik Köln
  • S. Fauser - Universitäts-Augenklinik Köln

German Retina Society. 24th Annual Conference of the German Retina Society. Aachen, 17.-18.06.2011. Düsseldorf: German Medical Science GMS Publishing House; 2011. Doc11rg06

doi: 10.3205/11rg06, urn:nbn:de:0183-11rg069

This is the English version of the article.
The German version can be found at: http://www.egms.de/de/meetings/rg2011/11rg06.shtml

Published: June 15, 2011

© 2011 Hörster et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

Outline

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Background: Retinal argon-laser-coagulation is an established procedure for induction of choroidal neovascularization (CNV) in rodents. This study aimed to evaluate in-vivo and ex vivo morphology and variability of CNV spots over time after laser treatment.

Methods: Female C57/Bl/6 mice, 3–5 months of age, were treated with five spots of retinal argon-laser coagulation per eye (150 mW, 100 ms, 50 µm). In-vivo fluorescence angiography (FA) and standard high-resolution spectral-domain optical coherence tomography (SD-OCT) were obtained at d0, 1, 4, 7, 14 and 21. Ex-vivo histology, CD31 immunostaining, flatmount and confocal microscopy were equally obtained. CNV size in retinal and choroidal focus, CNV morphology, central retinal thickness (CRT) and FA CNV activity grading were assessed at all time points and compared to ex-vivo appearance.

Results: SD-OCT revealed subretinal and intraretinal fluid and allowed evaluation of longitudinal morphologic changes of the induced CNV. Spot latitude and CRT did not differ significantly in longitudinal evaluation. However significant CNV activity changes were found in FA CNV activity grading, peaking at d4 and decreasing by d14. Assessment of CNV spot size by FA and SD-OCT showed significant correlation at timepoints with low activity and poor correlation at time points with highest activity.

Conclusion: Non-invasive SD-OCT provides additional morphologic information on laser-induced CNV. However, reliable evaluation of CNV activity requires FA. Spontaneous regression of CNV activity within 14 days after induction has to be taken into account when utilizing this model for testing efficacy of potential future treatments.