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129. Kongress der Deutschen Gesellschaft für Chirurgie

Deutsche Gesellschaft für Chirurgie

24.04. - 27.04.2012, Berlin

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Schwann cell metabolic activity in various short term holding conditions: implications for improved nerve graft viability

Meeting Abstract

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  • Christine Radtke - Medizinische Hochschule Hannover, Plastische, Hand- und Wiederherstellungschirurgie, Hannover
  • Insa Janssen - Medizinische Hochschule Hannover, Plastische, Hand- und Wiederherstellungschirurgie, Hannover
  • Kerstin Reimers - Medizinische Hochschule Hannover, Plastische, Hand- und Wiederherstellungschirurgie, Hannover
  • Christina Allmeling - Medizinische Hochschule Hannover, Plastische, Hand- und Wiederherstellungschirurgie, Hannover
  • Stella Maria Matthes - Medizinische Hochschule Hannover, Plastische, Hand- und Wiederherstellungschirurgie, Hannover
  • Peter M Vogt - Medizinische Hochschule Hannover, Plastische, Hand- und Wiederherstellungschirurgie, Hannover

Deutsche Gesellschaft für Chirurgie. 129. Kongress der Deutschen Gesellschaft für Chirurgie. Berlin, 24.-27.04.2012. Düsseldorf: German Medical Science GMS Publishing House; 2012. Doc12dgch167

doi: 10.3205/12dgch167, urn:nbn:de:0183-12dgch1675

Published: April 23, 2012

© 2012 Radtke et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

Outline

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Introduction: Strategies for improvement of nerve regeneration and optimal conditions to prevent Schwann cell (SC) loss within a nerve transplant procedure are critical. The purpose of this study was to examine SC viability, which play an important role in peripheral nerve regeneration, under various incubation conditions up to three hours. To address this issue, Schwann cell metabolic activity were determined using different independent test methods.

Materials and methods: The following experimental conditions were compared: SCs prepared from nerves were incubated in 1) isotonic saline solution 2) Dulbecco’s modified Eagles medium as used for cell culturing, 3) Hannover bioreactor medium and 4) Leibovitz’s medium. SC metabolic activity of excised rat sciatic nerve was determined at 4°C, 18°C and 37°C over 3 hrs.

Results: The results indicate that SC activity was optimized by usage of Leibovitz’s medium or HBRM at 37°C. Greater SC viability at the time surgical nerve grafting could contribute to improved axonal regeneration and remyelination after nerve transplantation, and thus more successful functional recovery.