gms | German Medical Science

25th Annual Meeting of the German Retina Society

German Retina Society

01.06. - 02.06.2012, Münster

Article

Send article

Early effects of triamcinolone acetonide on VEGF and PEDF in human choroidal neovascularization

Meeting Abstract

Search Medline for

  • Olcay Tatar - Universitäts-Augenklinik Tübingen
  • K. Shinoda - Laboratory of Visual Physiology, National Institute of Sensory Organs, Tokyo, Japan
  • A. Adam - Abteilung für Pathologie, Universität Tübingen
  • E. Kaiserling - Abteilung für Pathologie, Universität Tübingen
  • G. Pertile - Department of Ophthalmology, Sacro Cuore Hospital, Negrar, Italy
  • C. Eckardt - St. Joseph-Stift Augenklinik, Bremen
  • T. Eckert - St. Joseph-Stift Augenklinik, Bremen
  • P. Szurman - Universitäts-Augenklinik Tübingen
  • G. Scharioth - St. Joseph-Stift Augenklinik, Bremen
  • S. Bopp - MVZ Universitätsallee, Augenklinik, Bremen
  • C. Lücke - MVZ Universitätsallee, Augenklinik, Bremen
  • S. Grisanti - Universitäts-Augenklinik Lübeck
  • K.U. Bartz-Schmidt - Universitäts-Augenklinik Tübingen

German Retina Society. 25th Annual Conference of the German Retina Society. Münster, 01.-02.06.2012. Düsseldorf: German Medical Science GMS Publishing House; 2012. Doc12rg49

doi: 10.3205/12rg49, urn:nbn:de:0183-12rg497

This is the English version of the article.
The German version can be found at: http://www.egms.de/de/meetings/rg2012/12rg49.shtml

Published: May 30, 2012

© 2012 Tatar et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

Outline

Text

Objective: To evaluate the early effects of verteporfin photodynamic therapy (PDT) and triamcinolone acetonide (TA) combination therapy on pigment epithelium derived factor (PEDF) expression with regard to vascular endothelial growth factor (VEGF) in human choroidal neovascularization (CNV) secondary to age related macular degeneration.

Methods: Retrospective review of interventional series of 36 patients who underwent macular translocation with CNV extraction. Six patients were treated with intravitreal PDT-TA combination therapy (PDT-TA CNV) 3 to 9 days preoperatively. Twenty-six CNV without previous therapy (control CNV) and 4 CNV excised 3 days after PDT (PDT CNV) were used as control. CNV were stained for cytokeratin18, CD34, VEGF and PEDF.

Results: VEGF was stronger in retina pigment epithelium (RPE) (p=0.0002) and stroma of PDT-TA CNV (0.0103) and RPE of PDT CNV (p=0.0004) than control CNV. VEGF in PDT CNV and PDT-TA CNV did not show any significant change with respect to each other. PEDF was weaker in stroma of PDT CNV than in control CNV (p=0.01). PEDF expression in stroma of PDT-TA CNV was comparable to control CNV (p=0.9), however, was stronger than PDT CNV (p=0.03). VEGF predominance over PEDF was significantly higher in RPE of PDT CNV but not in PDT+TA CNV when compared to control CNV.

Conclusions: Administration of TA as adjuvant to PDT does not suppress the increase in VEGF expression but inhibits the decrease in PEDF expression, therefore prevents VEGF predominance over PEDF early after PDT.